Residual Porcine Genomic DNA Probe-Based qPCR Kit (No Internal Control), 50 Tests/Box (CAT#: STEM-SLSR-1454-ZJF)

This real-time PCR detection kit is based on probe technology and is designed to identify Residual Porcine Genomic DNA (No Internal Control) through specific amplification of conserved gene regions. Fluorescent signals generated during the PCR process are detected in real time, enabling precise qualitative and quantitative assessment of Residual Porcine Genomic DNA (No Internal Control) DNA in various sample types.

Kit Components:
Residual Porcine Genomic DNA (No Internal Control) Amplification Mix: 50 µL × 1 tube
Residual Porcine Genomic DNA (No Internal Control) Reaction Mix: 950 µL × 1 tube
Residual Porcine Genomic DNA (No Internal Control) Positive Control: 50 µL × 1 tube
Residual Porcine Genomic DNA (No Internal Control) Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: Reagents in the kit are sufficient for the number of tests indicated on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light, avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
Compatible with real-time PCR systems including ABI, MX3000P/3005P, LightCycler, Bio-Rad, and Eppendorf.
Additional required equipment: DNase/RNase-free 1.5 mL centrifuge tubes, 0.2 mL PCR reaction tubes, filter tips (10 µL, 200 µL, 1000 µL), centrifuge, vortex mixer, and pipettes of various capacities.
Sample Requirements:
Depending on circumstances, use intestinal tissue from dead or culled cattle under sterile conditions. For live animals, samples may include whole blood, serum, plasma, feces, vomit, oral/nasopharyngeal/respiratory secretions, throat/nasal/ocular/anal swabs, or viral cultures.
Cross-contamination must be avoided. Samples should be tested promptly after collection or stored at -20°C ± 5°C. For long-term storage, samples should be kept below -70°C.
Sample Storage and Transport:
Short-term: Store at -20°C.
Long-term (up to 6 months): Store below -70°C.
Transport using 2-8°C ice packs. Repeated freeze-thaw cycles are strictly prohibited.

No need for additional preparation; add DNA and start.
Specific detection enabled by primers/probes based on conserved sequences.
Assay components are fine-tuned for high sensitivity.
Positive control included to verify test reliability.
Closed-system qPCR protocol helps prevent contamination.
Time-efficient workflow for quick results.
Intended solely for scientific investigations.

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