Quench-Flow Technique (CAT#: STEM-AC-0030-WXH)

Name: Quench-Flow Technique
SKU: STEM-AC-0030-WXH
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Introduction

Rapid quench-flow is a well established kinetic technique where the experimentalist can isolate intermediates and products within a reaction mechanism and analyse them by analytical methods providing a means of specifically identifying these components. This is a complementary technique to stopped-flow and can be used when the reaction does not contain spectroscopic changes or changes cannot identify specific reaction components.

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Principle Applications Procedure Materials

Principle

The principle is to mix the two solutions and then to observe a change in spectroscopic properties of the mixture at different place along the reaction tube during the flow. In the continuous mode of the quenched-flow method, the observation chamber is replaced by a second mixer in which the quenching agent arrives.

Applications

Used to determine fast reaction rates or single turnover rates of enzymatic reactions and to isolate reaction intermediates.

Procedure

Small volumes of solutions are driven through a high efficiency mixer and flow into a delay (or ageing) loop. After a set time, the reaction is stopped (or quenched) by the addition of a chemical quench solution.