ProteoSure™ Recombinant Endoproteinase Lys-C, Sequencing Grade, 2 x 20 μg, 14-LC-040UG, Marvelgent biosciences (CAT#: STEM-GT-3051-LGZ)

The endoprotease Lys-C is a serine protease that specifically cleaves amide and peptide ester bonds on the carboxyl side of lysine and s-aminoethylcysteine residues.
Recombinant Endoprotease Lys-C is a genetically engineered lysine endoprotease from Achromobacter lyticus. It was expressed in E. coli and purified by high pressure liquid chromatography (HPLC).

Recommended Use
The recommended ratio to sample protein is 1:1000 ~ 1:20,000 (w/w), and the optimum pH is pH 7 ~ 9.5. Enzymatic reactions should be performed at 30 - 37°C for 2 - 24 hours.
Enzyme inhibitors (such as aprotinin, DFP, leupeptin, and TLCK) or denaturation of proteins with guanidine chloride (GuCl) are not recommended.

Recombinant Lys-C is compatible with the following reagents: 0.2% SDS, 1.0% Triton, 6M urea, 0.5 M NaCl and 20% acetonitrile.

Storage and Stability
Recombinant carboxypeptidase B freeze-dried powder is stored in a sealed container at a storage temperature of 2-8°C. Stable for 12 months.
The lyophilized powder can be reconstituted to a working concentration with ddH2O; the solution is stable for 7 days at 2-8°C.
It is also recommended to dissolve the lyophilized powder in 25 mM Tris-HCl (pH 8.5) and make a stock solution concentrate. Aliquots of the stock solution can be stored at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles.

Size: 2 x 20 μg
CAS #: 78642-25-8
Enzyme Commission #: 3.4.21.50
Molecular weight: 31 kDa
Origin: Achromobacter lyticus
Source: E.coli
Purified by: HPLC
Physical form: Lyophilized powder
Specific activity: ≥ 3.0 AU/mg protein
Unit definition: One Amidase Unit (AU) is the amount of enzyme that produces 1 micromole Ac-Lys-pNA per minute at 25 mM Tris-HCl (pH 9.0) at 37°C.
Contaminant activity: No trypsin, chymotrypsin, carboxypeptidase A, Lys-N, Arg-C or other protease contaminants.
Storage: Store in a sealed container at 2 - 8°C. Once reconstituted, it should be stored at -20°C for up to 6 months. Avoid freeze-and-thaw cycles.
Intended use: For research only. Not intended for any human or animal diagnostic or therapeutic use.

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