Plasmid Seplife LX Affinity Medium, ≥3 mg Plasmid DNA/mL (CAT#: STEM-BC-3082-ZJF)

This affinity chromatography medium is designed for purification of supercoiled plasmid DNA. It uses highly crosslinked agarose microspheres functionalized with a thiophilic aromatic ligand. The pyridyl-thiol ligand provides selective interactions suitable for plasmid purification. The medium is intended for gene-therapy and DNA-vaccine development workflows. Small, uniform particles support chromatographic resolution and efficient mass transfer. A dynamic binding capacity of at least 3 mg/mL supports preparative plasmid processing. The hydrophilic matrix limits nonspecific adsorption during purification. Broad pH and chemical stability support repeated use and cleaning-in-place. The medium can be scaled from development columns to larger production systems.

Model: Plasmid Seplife LX
Appearance: White spherical particles, odorless and tasteless
Matrix: Highly crosslinked agarose microspheres
Ligand: Pyridyl thiol
Ligand density: 27-50 µmol/mL
Median particle size d50v: 36-44 µm
Dynamic binding capacity: ≥3 mg/mL
Operating temperature: 4-30°C
Flow performance: 220 cm/h at 20 cm bed height and 0.3 MPa
Long-term pH stability: 3-13
Short-term CIP pH stability: 2-14
Chemical compatibility: Common aqueous buffers, 1 mol/L sodium hydroxide, 70% ethanol, 40% isopropanol, and 1 M acetic acid
Storage: 20% ethanol at 4-30°C

Selective supercoiled plasmid purification
High binding capacity
Small high-resolution particles
Hydrophilic agarose matrix
Low nonspecific adsorption
Broad CIP compatibility
High-flow process operation
Scalable gene-therapy purification

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