Phenyl Seplife FF LS Hydrophobic Interaction Medium, 33 mg HSA/mL (CAT#: STEM-BC-3141-ZJF)

Highlights

Phenyl hydrophobic ligand
Optimized for monoclonal antibodies
33 mg/mL HSA capacity

Cat Number: STEM-BC-3141-ZJF

Application: for monoclonal antibody hydrophobic purification

Model: Phenyl Seplife FF(LS)




Description

This hydrophobic interaction chromatography medium is designed for purification of monoclonal antibodies. It uses a high-flow agarose matrix functionalized with phenyl ligands. The ligands interact with hydrophobic regions exposed on biological molecules. Binding is promoted under elevated salt conditions. Reducing the salt concentration releases molecules according to their hydrophobic interaction strength. The lower ligand density provides controlled hydrophobic selectivity for antibody purification. Spherical particles support uniform column packing and reproducible chromatographic operation. Broad pH and chemical stability support repeated processing and cleaning-in-place.

Specification

Model: Phenyl Seplife FF(LS)
Appearance: White spherical gel, odorless and tasteless
Matrix: Seplife 6FF
Ligand: Phenyl
Shape: Spherical
Particle size: 45-165 µm
Maximum flow rate: 600 cm/h
Pressure resistance: 0.3 MPa
Operating temperature: 4-40°C
Ligand density: 25 µmol phenyl/mL medium
Protein adsorption capacity: 33 mg HSA/mL medium
Long-term pH stability: 3-13
Short-term CIP pH stability: 2-14
Chemical compatibility: 1 mol/L sodium hydroxide, 70% ethanol, 30% isopropanol, 0.5% SDS, 10% ethylene glycol, 6 mol/L guanidine hydrochloride, 8 mol/L urea, and 3 mol/L ammonium sulfate

Features

Phenyl hydrophobic ligand
Optimized for monoclonal antibodies
33 mg/mL HSA capacity
Up to 600 cm/h flow
High-flow agarose matrix
Broad chemical compatibility
Wide CIP pH range
Uniform spherical particles

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