Phenyl Large Scale HP Hydrophobic Interaction Medium, ~40 µm (CAT#: STEM-BC-3135-ZJF)

This high-performance hydrophobic interaction medium is designed for purification of biological macromolecules. It uses small high-flow agarose microspheres functionalized with phenyl ligands. Strong hydrophobic interactions provide selectivity based on differences in biomolecule surface hydrophobicity. Samples bind under elevated salt concentration and elute as the salt concentration is reduced. The approximately 40 µm particles improve chromatographic resolution and mass transfer. Dynamic HSA capacity above 19 mg/mL supports preparative purification. The medium operates above 220 cm/h at the specified pressure and bed height. Broad pH stability permits cleaning-in-place across an extended range. Its chemical compatibility supports repeated use with common denaturants, alcohols, detergents, and alkaline cleaning solutions.

Model: Phenyl Large Scale HP
Appearance: White spherical gel, odorless and tasteless
Type: Strong hydrophobic interaction medium
Matrix: Large Scale HP microspheres
Ligand: Phenyl
Median particle size d50v: Approximately 40 µm
Dynamic binding capacity QB10:>19 mg HSA/mL
Ligand density:>9 µmol/mL
Operating temperature: 4-30°C
Flow performance:>220 cm/h at 0.3 MPa differential pressure and 20 cm bed height
Long-term pH stability: 3-13
Short-term CIP pH stability: 2-14
Chemical compatibility: 1 mol/L sodium hydroxide, 70% ethanol, 30% isopropanol, 0.5% SDS, 10% ethylene glycol, 6 mol/L guanidine hydrochloride, 8 mol/L urea, and 3 mol/L ammonium sulfate

High-resolution 40 µm particles
Strong phenyl hydrophobicity
>19 mg/mL HSA capacity
High-flow agarose matrix
Broad CIP pH range
Strong chemical resistance
Efficient mass transfer
Suitable for polishing purification

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