Pertussis Bordetella Probe-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-2425-ZJF)

Using high-performance probes and precision-designed primers, this real-time PCR kit enables the detection of Pertussis Bordetella by targeting conserved genomic regions. Fluorescence signals are emitted as the target is amplified, and the system records data in real time for confident qualitative and quantitative evaluation.

Kit Components:
Pertussis Bordetella Amplification Mix: 50 µL × 1 tube
Pertussis Bordetella Reaction Mix: 950 µL × 1 tube
Pertussis Bordetella Positive Control: 50 µL × 1 tube
Pertussis Bordetella Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: Reagents in the kit are sufficient for the number of tests indicated on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light, avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
Compatible with real-time PCR systems including ABI, MX3000P/3005P, LightCycler, Bio-Rad, and Eppendorf.
Additional required equipment: DNase/RNase-free 1.5 mL centrifuge tubes, 0.2 mL PCR reaction tubes, filter tips (10 µL, 200 µL, 1000 µL), centrifuge, vortex mixer, and pipettes of various capacities.
Sample Requirements:
Depending on circumstances, use intestinal tissue from dead or culled cattle under sterile conditions. For live animals, samples may include whole blood, serum, plasma, feces, vomit, oral/nasopharyngeal/respiratory secretions, throat/nasal/ocular/anal swabs, or viral cultures.
Cross-contamination must be avoided. Samples should be tested promptly after collection or stored at -20°C ± 5°C. For long-term storage, samples should be kept below -70°C.
Sample Storage and Transport:
Short-term: Store at -20°C.
Long-term (up to 6 months): Store below -70°C.
Transport using 2-8°C ice packs. Repeated freeze-thaw cycles are strictly prohibited.

Simplified operation with pre-mixed reagents requiring only DNA input.
Uses conserved region alignment to ensure high specificity.
Detects low-copy targets with high sensitivity due to optimized chemistry.
Contains a positive control for result validation.
Enclosed one-tube protocol limits contamination.
Fast turnaround for time-sensitive applications.
Restricted to laboratory research applications.

STEM-SLSR-2423-ZJF
STEM-SLSR-2424-ZJF
STEM-SLSR-2426-ZJF
STEM-SLSR-2427-ZJF