Patch-clamp Technique to study electrophysiological properties of biological membranes (CAT#: STEM-PET-0019-WXH)

Introduction

The patch-clamp technique is a powerful technichnique to study electrophysiological properties of biological membranes. It allows for recording of macroscopic whole-cell and microscopic single-channel currents flowing through ion channels.<br />Central to the patch-clamp technique is the patch-clamp pipette. It is a thin glass tube, which is pulled out to a width of only some micrometers. It is filled with electrolyte solution, into which a Ag/AgCl electrode is immersed. When the patch-clamp pipette is brought into close contact to the cell membrane, a tight connection between the pipette and the cell membrane forms. The seal can be enhanced by applying suction to the pipette, so that the resistance to the bath solution exceeds several GΩ.

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Principle Applications Procedure Materials Notes Related Products

Applications

• Characterization of the properties of cell membrane ion channels and their kinetic studies.
• Single cell morphology and function studies.
• Studies on the mechanism of drug action.
• Studies of cellular secretion.
• Studies of signal transduction.
• Studies of molecular biology.

Procedure

1.Prepare solution
2.Prepare cells or brain slices
3.Pull and polish the pipette
4.Set up the perfusion system
5.Patching a cell
6.Signal acquisition and amplification
7.Signal digitization
8.Data acquisition and analysis

Notes

Customer provided:
1. Testing requirements and reference materials.
2. Experimental model/sample.
3. Analytical method.
4. Raw data.

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