Pasteurella multocida (Rabbit) Probe-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-1664-ZJF)

This real-time PCR kit is designed for the sensitive and specific detection of Pasteurella multocida (Rabbit) using probe-based technology. Primers targeting conserved gene regions of Pasteurella multocida (Rabbit) ensure precise amplification. The generated fluorescence is continuously tracked throughout the reaction, enabling reliable qualitative and quantitative diagnostics.

Kit Components:
Pasteurella multocida (Rabbit) Amplification Mix: 50 µL × 1 tube
Pasteurella multocida (Rabbit) Reaction Mix: 950 µL × 1 tube
Pasteurella multocida (Rabbit) Positive Control: 50 µL × 1 tube
Pasteurella multocida (Rabbit) Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: Reagents in the kit are sufficient for the number of tests indicated on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light, avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
Compatible with real-time PCR systems including ABI, MX3000P/3005P, LightCycler, Bio-Rad, and Eppendorf.
Additional required equipment: DNase/RNase-free 1.5 mL centrifuge tubes, 0.2 mL PCR reaction tubes, filter tips (10 µL, 200 µL, 1000 µL), centrifuge, vortex mixer, and pipettes of various capacities.
Sample Requirements:
Depending on circumstances, use intestinal tissue from dead or culled cattle under sterile conditions. For live animals, samples may include whole blood, serum, plasma, feces, vomit, oral/nasopharyngeal/respiratory secretions, throat/nasal/ocular/anal swabs, or viral cultures.
Cross-contamination must be avoided. Samples should be tested promptly after collection or stored at -20°C ± 5°C. For long-term storage, samples should be kept below -70°C.
Sample Storage and Transport:
Short-term: Store at -20°C.
Long-term (up to 6 months): Store below -70°C.
Transport using 2-8°C ice packs. Repeated freeze-thaw cycles are strictly prohibited.

No need for additional preparation; add DNA and start.
Specific detection enabled by primers/probes based on conserved sequences.
Assay components are fine-tuned for high sensitivity.
Positive control included to verify test reliability.
Closed-system qPCR protocol helps prevent contamination.
Time-efficient workflow for quick results.
Intended solely for scientific investigations.

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