Nuclear System Yeast Library Construction to study protein-protein interactions (CAT#: STEM-MB-0015-WXH)

Introduction

The nuclear-protein yeast two-hybrid technique was first established by Fields et al. when studying the nature of yeast transcription factor GAL4, and has subsequently developed into a mature protein-protein interaction research tool with the characteristics of simplicity, sensitivity and reflecting the real situation of protein interactions in living cells. <br />Currently, there are two methods for yeast library construction, one is the SMART system, which adds a unique step to the existing yeast two-hybrid library construction process (BD MatchmakerTM library), resulting in a significant reduction in the high abundance of genes expressed in the library, thus making the library uniform in abundance and increasing the screening positivity rate; the second is the Gateway system, which uses the Gateway site-specific The second is the Gateway system, which uses the Gateway site-specific recombination technology (Cloneminer cDNA library construction kit) to construct libraries.