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Morphological study of cartilage and bone by transmission electron microscopy technology (CAT#: STEM-MIT-0003-LJX)

Introduction

Cartilage, or cartilage tissue, is made up of chondrocytes and intercellular matter. The matrix in the cartilage is gel and has greater toughness. Cartilage is a connective tissue that is primarily supportive. Cartilage does not contain blood vessels and lymphatic vessels, nutrients from the blood vessels in the perichondrium penetrate into the intercellular stroma, and then nourish bone cells.<br />Bone cells are the main cells in mature bone tissue, which is equivalent to the adult stage of human, and are transformed from osteoblasts. When the new bone matrix calcifies, the cells become embedded in it. At this point, the synthesis of cells stops, and the cytoplasm is reduced, becoming bone cells. Bone cells play a role in both bone resorption and bone formation and are the main cells that maintain the metabolism of mature bone.




Principle

Transmission electron microscopy (TEM) is to project the accelerated and concentrated electron beam onto a very thin sample, and the electron collides with the atoms in the sample and changes the direction, thus generating the stereo scattering Angle. The size of the scattering Angle is related to the density and thickness of the sample, so the image can be formed with different shades. The image can be enlarged, focused and displayed on imaging devices such as fluorescent screens, film and photosensitive coupling components. The resolution of transmission electron microscope is much higher than that of optical microscope, can reach 0.1~0.3nm, magnification of tens of thousands to millions of times. Therefore, transmission electron microscopy can be used to observe the fine structure of the sample.

Applications

Microscopic imaging in materials science or biology.

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Cartilage and bone cells

Notes

Pay attention to air humidity
Voltage needs to be stabilized