Monitor real-time alpha-synuclein aggregation by Dual polarization interferometry (DPI) (CAT#: STEM-MB-0412-WXH)

Name: Monitor real-time alpha-synuclein aggregation by Dual polarization interferometry (DPI)
SKU: STEM-MB-0412-WXH
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Introduction

Alpha-synuclein (aSyn) is a protein that, in humans, is encoded by the SNCA gene. It is a neuronal protein that regulates synaptic vesicle trafficking and subsequent neurotransmitter release.
Alpha-synuclein (αS) aggregation participates in neurodegenerative maladies including Parkinson's disease (PD), multiple system atrophy, and dementia with Lewy bodies

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Principle Applications Procedure Materials

Principle

Dual polarization interferometry (DPI) is an analytical technique that allows the simultaneous determination of thickness, density, and mass of a biological layer on a sensing waveguide surface in real time. DPI focuses laser light into two waveguides. One of these functions as the "sensing" waveguide having an exposed surface while the second one functions to maintain a reference beam. A two-dimensional interference pattern is formed in the far field by combining the light passing through the two waveguides. The DPI technique rotates the polarization of the laser, to alternately excite two polarization modes of the waveguides. Measurement of the interferogram for both polarizations allows both the refractive index and the thickness of the adsorbed layer to be calculated. These measurements can be used to infer conformational information about the molecular interactions taking place, as the molecule size (from the layer thickness) and the fold density (from the RI) change.

Applications

Monitor real-time alpha-synuclein aggregation.
Study of the mechanism of neurodegenerative maladies.
Drug Discovery

Procedure

1. Setting of dual polarization interferometry
2. Preparing the DPI sensor chip
3. Immobilization of target on DPI biosensor
4. Reagent was injected to react
5. Quantitative analysis