MicroSEQ™ Full Gene 16S rDNA Sequencing Kit, MicroSEQ™, Thermo Fisher Scientific (CAT#: STEM-AS-0287-LSY)

MicroSEQ™ Full Gene 16S rDNA Sequencing Kit, MicroSEQ™, model 4347484 is manufactured by Thermo Fisher Scientific. The MicroSEQ® 16S rDNA sequencing kit is the sequencing component of the MicroSEQ® 16S rDNA full gene identification system, which provides an easy-to-use method based on DNA sequence to identify most bacteria. Use this kit to sequence the PCR products generated by MicroSEQ®Full Gene 16S rDNA PCR Kit. Together, these two kits can quickly and easily amplify and sequence bacterial 16S rRNA genes. For research use only. Not suitable for diagnostic procedures. The kit includes protocol and quick reference card. The sequencing kit does not include a protocol or quick reference card.
AppliedBiosystems®MicroSEQ®System simplifies each step of microbial identification, combining the advantages of MicroSEQ ID software, simple PrepMan Ultra sample preparation reagents and protocols, MicroSEQ® bacteria and fungi application kits, and industry-leading thermal cyclers and sequencing systems. To ensure the best performance and system-wide integration, we develop and test all components of the system together.

Applies to (equipment): 310 Genetic Analyzer, 3100 Genetic Analyzer, 3100-Avant Genetic Analyzer, 3130xl Genetic Analyzer, 3730xl DNA Analyzer, GeneAmp 9700, 3730 DNA Analyzer, 3500xL Genetic Analyzer, 3500 Genetic Analyzer
Sample type: PCR Products
Detection Method: Capillary Electrophoresis
Software: MicroSEQ® ID Analysis Software
Library: Customizable, Bacterial Library (> 2000 species)
Shipping Condition: Dry Ice
Target Molecule: DNA
Target Gene: 16S rRNA (full gene)
Target Organism Class: Bacteria
Technology: SBT (Sequence-Based Typing)
Validated Application: Sequencing
Workflow Step: Cycle Sequencing
Product Line: MicroSEQ™
Number: 4347484
Unit specifications: 1 kit
Contents: Each kit contains three tubes each of forward and reverse sequencing mix. Sufficient reagent is supplied to perform 15 forward and 15 reverse sequencing reactions of the 16s rRNA gene.
Storage: Store at -15 to -25°C.

Sequence-based measurements ensure that you get repeatable results within 24 hours.
Measurements that are not related to growth requirements allow you to use objective criteria to identify bacteria.
Universal domain-level primers eliminate pre-optimization.
MicroSEQ ID analysis software ensures accurate species identification.
Customizable libraries give you the flexibility to create new libraries and search for verified libraries containing the most frequently encountered creatures.
Compared with MicroSEQ®D2 LSU Fungal Kit and MicroSeq®500 16S rDNA Kit, it requires minimal training and similar workflow.

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