MicroSEQ™ D2 rDNA Fungal Sequencing Kit, MicroSEQ™, Thermo Fisher Scientific (CAT#: STEM-AS-0288-LSY)

MicroSEQ™ D2 rDNA Fungal Sequencing Kit, MicroSEQ™, model 4347481 is manufactured by Thermo Fisher Scientific. This kit is the sequencing component of the MicroSEQ® Fungi Identification System, and provides an easy-to-use method based on DNA sequence to identify most fungi. It includes the primers needed to sequence the PCR products generated using the PCR components. The agreement and quick reference card are not included. For research use only. Not suitable for diagnostic procedures.
Based on the phylogenetic analysis of the D2 region of the large subunit ribosomal RNA gene sequence, this easy-to-use kit allows you to identify even the most difficult fungal, yeast, and oomycete pathogens. To ensure accurate and specific results, the software of the kit compares your samples with D2 sequence databases from more than 1,000 validated species.
AppliedBiosystems®MicroSEQ®System simplifies each step of microbial identification, combining the advantages of MicroSEQ®ID software, simple PrepMan Ultra sample preparation reagents and protocols, MicroSEQ® bacteria and fungi application kits, and industry-leading thermal cyclers and sequencing systems. To ensure the best performance and system-wide integration, we develop and test all components of the system together.

Applies to (equipment): 310 Genetic Analyzer, 3100 Genetic Analyzer, 3100-Avant Genetic Analyzer, 3130xl Genetic Analyzer, 3730xl DNA Analyzer, GeneAmp 9700, Veriti Thermal Cycler, 3500 Genetic Analyzer, 3730 DNA Analyzer, 3500xL Genetic Analyzer, 9800 Fast Thermal Cycler
Sample type: PCR Products
Detection Method: Capillary Electrophoresis
Software: MicroSEQ® ID Analysis Software
Library: Fungal Library (> 1100 species), Customizable
Shipping Condition: Dry Ice
Target Molecule: DNA
Target Gene: D2 region of rRNA gene
Target Organism Class: Fungi
Technology: SBT (Sequence-Based Typing)
Validated Application: Sequencing
Workflow Step: Cycle Sequencing
Product Line: MicroSEQ™
Number: 4347481
Unit specifications: 1 kit
Contents and storage:
The kit contains reagents sufficient for 55 sequencing reactions:
D2 Forward Sequencing Mix (store at -15 to -25°C)
D2 Reverse Sequencing Mix (store at -15 to -25°C)

Sequence-based measurements ensure that you get repeatable results in less than 24 hours.
Measurements that are independent of growth requirements allow you to use objective criteria to identify fungi.
Universal domain-level primers eliminate pre-optimization.
MicroSEQ ID analysis software ensures accurate species identification.
Customizable libraries give you the flexibility to create new libraries and search for verified libraries containing the most frequently encountered creatures.
The minimum training is required, and the workflow is the same as the MicroSEQ®Full Gene 16S rDNA bacterial identification kit.

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