Measurement of Plasma Viscosity by Rotating Viscometer Method (CAT#: STEM-PPA-0016-YJL)

Introduction

Tissue damage induces a local inflammatory response that in turn stimulates, through interleukin and other mediators, an increase in hepatic synthesis of several acute phase proteins. Quantitative assay of these acute phase proteins in serum is useful for diagnosing and monitoring an inflammatory response. The more complex hyperproteinaemia of the later stages of inflammation (> 24 hours) should be measured by tests such as the erythrocyte sedimentation rate (ESR) and plasma viscosity, which are sensitive to the combined effect of multiple proteins. <br />Measurement of plasma viscosity has several advantages over measurement of ESR. These include independence from the effects of anaemia, polycythaemia, and erythrocyte deformability; a reference range that is independent of sex and less dependent on age; a shorter assay time; the facility to store plasma for subsequent assay; and the potential for calibration, quality control, and automation.

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Principle Applications Procedure Materials Notes Related Products

Principle

The principle of the rotating viscometer method to measure the force acting on a rotor (torque) when it rotates at a constant angular velocity (rotational speed) in a liquid. Rotating viscometers are used for measuring the viscosity of Newtonian (shear-independent viscosity) or non-Newtonian liquids (shear dependent viscosity or apparent viscosity). Rotating viscometers can be divided in 2 groups, namely absolute and relative viscometers. In absolute viscometers the flow in the measuring geometry is well defined.
The measurements result in absolute viscosity values, which can be compared with any other absolute values. In relative viscometers the flow in the measuring geometry is not defined. The measurements result in relative viscosity values, which cannot be compared with absolute values or other relative values if not determined by the same relative viscometer method. Different measuring systems are available for given viscosity ranges as well as several rotational speeds.

Applications

Mineral oil industry; Food industry; Cosmetic/pharmaceutical industry; Petroleum industry; Chemical industry

Procedure

1. Pour the liquid into the measuring cup.
2. Insert the spindle into the liquid.
3. Rotate the spindle and measure the resistance.

Materials

• Sample Type: liquid, gel-like, or semi-solid everyday substances

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