Measurement of Enzyme Kinetics by Continuous-Flow Analysis (CAT#: STEM-AC-0003-WXH)

Name: Measurement of Enzyme Kinetics by Continuous-Flow Analysis
SKU: STEM-AC-0003-WXH
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Introduction

Enzyme kinetics is the study of the rates of enzyme-catalysed chemical reactions. In enzyme kinetics, the reaction rate is measured and the effects of varying the conditions of the reaction are investigated. Studying an enzyme's kinetics in this way can reveal the catalytic mechanism of this enzyme, its role in metabolism, how its activity is controlled, and how a drug or a modifier (inhibitor or activator) might affect the rate.

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Principle Applications Procedure Materials

Principle

In continuous flow analysis (CFA), a sample is injected into a flowing carrier solution passing rapidly through small-bore tubing. The sample is mixed with a reagent, which reacts with the sample to develop a color and determine the sample concentration. The use of carefully controlled flow conditions ensures that the color development reaction is reproducible, so that the color measurement need not wait until the reaction has gone to completion.

Applications

Study fast reactions consists of mixing two or more reagents, suitable for water, food, grain, oil, beer, wine, tobacco and other fields.

Procedure

1. Using continuous flow, the sample solution is aspirated from various reagent containers, and the sample and reagent are mixed in the flow path system.
2. Using a peristaltic pump to inhale air, samples and reagents into the determined flow path in a specific order and ratio, and finally reach the detector to complete the analysis process of detection.