LS 50B Spectrometer, Perkin Elmer (CAT#: STEM-CTEPO-0103-ZJF)

The manufacturer of the LS 50B Spectrometer, Perkin Elmer is Perkin Elmer.
Refurbished Perkin Elmer LS-50B Spectrometer (p/n L225-0115) complete with stirred 4-position cell changer compartment and an additional microplate transport assembly compartment. Also, including Dell Optiplex GX270 with PE FL Winlab V4.00.02, Microsoft Windows 2000 Professional and Dell 17" LCD monitor.
The scanning range is 200-900 nm. Gated photomultiplier with modified S5 response for operation to about 650 nm. Interchangeable red-sensitive photomultiplier available for use to 900 nm. Wavelength Accuracy is plus or minus 1.0nm.
The Perkin-Elmer LS50B Luminescence Spectrometer is a complete computer controlled ratioing luminescence spectrometer with the capability of measuring fluorescence, phosphorescence, chemiluminescence and bioluminescence. Featuring excitation and emission monochromators as well as excitation/emission polarizers suitable for fluorescence polarization, anisotropy, and ratio measurements, this instrument is routinely used for obtaining information regarding kinetics in competitive ligand binding assays on a millisecond timescale.

Condition: Refurbished, Certified Pre-owned
Principle: Computer controlled ratioing luminescence spectrometer with the capability of measuring fluorescence, phosphorescence, chemiluminescence and bioluminescence.
Source: Xenon discharge lamp, equivalent to 20 kW for 8 µs duration. Pulse width at half height <10 µs.
Sample detector: Gated photomultiplier with modified S5 response for operation up to around 650 nm. Red-sensitive R928 photomultiplier can be optionally fitted for operation up to 900 nm.
Reference detector: Gated photomultiplier with modified S5 response for operation up to around 650 nm.
Monochromators: Monk-Gillieson type monochromators cover the following ranges:
Excitation 200-800 nm with zero order selectable.
Emission 200-650 nm with standard photomultiplier with zero order selectable, 200-900 nm with optional R928 photomultiplier. Synchronous scanning is available with constant wavelength or constant energy difference.
Wavelength accuracy: +1.0 nm
Wavelength reproducibility: +0.5 nm
Spectral bandpass: The excitation slits (2.5-15.0 nm) and emission slits (2.5-20.0 nm) can be varied and selected in 0.1 nm increments.
Phosphorescence mode: Delay and gate times can be varied to measure phosphorescence. Minimum total period 13.0 ms (50 Hz)
Scanning speed: Scanning speed can be selected in increments of 1 nm for 10-1500 nm/min. Time-dependent data can also be collected.
Emission filters: Computer selectable cut-off (high-pass) filters at 290, 350, 390, 430 and 515 nm, a blank (to act as shutter), a 1% T attenuator and clear beam position.
Sensitivity: Signal to noise is 500: 1 r.m.s., using the Raman band of water with excitation at 350 nm, excitation and emission bandpass 10 nm.
Standard cell holder: A single position water thermostattable holder for 10 mm cuvettes.

Scanning range is 200-900 nm.
Gated photomultiplier with modified S5 response for operation to about 650 nm.
Interchangeable red-sensitive photomultiplier available for use to 900 nm.
Wavelength Accuracy is plus or minus 1.0nm.

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