Long-Range PCR (CAT#: STEM-MB-0211-WXH)

Long-range PCR can be achieved by using modified high-efficiency polymerases with enhanced DNA binding, resulting in highly processive and accurate amplification of long fragments.

• Targeted resequencing and genotyping studies involving detection of gross alterations of the genome such as large deletions or substitutions.
• Genomic fingerprinting for screening of diseases.
• Validation of copy number variants (CNVs) in biomedical research.

1.Primer design
2.Preparation of template
3.PCR reaction system and reaction parameters set
4.Detection of amplification products

1.fidelity Taq DNA polymerase: Vent polymerase (a thermostable archean DNA polymerase ), A combination of two different polymerases (one with high concentration, normally the Taq DNA polymerase and another one with low concentration, a high fidelity DNA polymerase.)
2.Primer:
The primers used in the long PCR are 22 to 30 nucleotides long and 50% of less GC content in it. Furthermore, it should have a low capacity of secondary structure formation and annealing temperature between 58 ̊C to 68 ̊C.