Listeria Monocytogenes Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0621-ZJF)

This assay kit applies SYBR Green dye-based real-time PCR to amplify and detect Listeria Monocytogenes nucleic acids. Specific primers are designed to target conserved regions of the target gene. Fluorescence signals emitted during amplification are recorded in real time, supporting accurate identification and measurement of Listeria Monocytogenes in experimental and routine laboratory settings.

Kit Components:
Listeria Monocytogenes Amplification Mix: 50 µL × 1 tube
Listeria Monocytogenes Reaction Mix: 950 µL × 1 tube
Listeria Monocytogenes Positive Control: 50 µL × 1 tube
Listeria Monocytogenes Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Designed for immediate use with user-provided DNA.
Selective primers crafted from highly conserved genetic regions.
Fine-tuned probe and primer chemistry enhances detection power.
Includes control sample for clear interpretation.
Sealed fluorescence PCR tube reduces contamination possibilities.
For investigational use in laboratories.

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