Life Tech Attune NxT Flow cytometer, Dell Flat Panel LCD Monitor, Life Tech Attune (CAT#: STEM-CAPO-0007-LGZ)

The Attune NxT can be designed to accommodate the most common fluorophores and fluorescent proteins used in flow cytometry to match most panels you are currently running. A variety of fluorescent proteins can be detected with an (optional) 561 nm laser. The Attune NxT flow cytometer, designed to use acoustically assisted hydrodynamic focusing, achieves sample throughputs of 12.5 μL/min - 1 mL/min or 12.5 - 1000 μL/min 10 times faster than conventional hydrodynamic focusing systems, The data acquisition rate was 35,000 events/sec. Acoustic focusing significantly increases sampling rate, which simplifies sample preparation, reduces protocol time, and minimizes cell loss. This feature is suitable for wash-free, lysis-free immunophenotyping protocols using whole blood and samples with lower intrinsic concentrations, including cerebrospinal fluid and stem cell preparations. As a personal flow cytometer, the Attune NxT AFC2 matches the most sophisticated multi-laser, multi-color instruments on the market today and meets the most complex experimental needs.

Condition: certified pre-owned
Lasers: Violet: 405 nM (50 mW); Blue: 488 nM (50 mW); Yellow: 561 nM (50 mW); Red: 637 nM (100 mW)
Filters: VL1: 440/50; VL2: 512/25; VL3: 603/48; VL4: 710/50; RL1: 670/14; RL2: 720/30; RL3: 780/60; YL1: 585/16; YL2: 620/15; YL3: 695/40; YL4: 780/60; BL1: 530/30; BL2: 590/40; BL3: 695/40
Scatter: Forward scatter: Photodiode detector with 488/10; Side scatter: PMT with 488/10
Control and Analysis: Dell Desktop with Intel CORE i7 with Windows 7; Attune NxT Software version 2.2; Dell Flat Panel LCD monitor
Automated maintenance: startup (less than 15 min), shutdown, rinse, wash, debubble, deep clean, monthly decontamination
Gates: standard and customizable gates
Graphics resolution: publication-quality images (customizable)
Probe Design: Intelligent probe design minimizes clogging and carryover (less than 1%) – helps prevent damage to the instrument
Sample Mixing: Mixes sample by aspiration to maintain sample homogeneity and cell viability
Laser: Simmer mode: automatic shutoff prolongs laser usage lifetime by 10xPre-aligned and welded fiber to laser interfacePre-aligned fiber to beam-shaping optics (BSO) interface (alignment-free)All lasers spatially separatedFocus lens per laser: power where you want itFlat-top laser specified at the flow cell Benefits of flat-top lasers
• ~50% reduction in scattered light
• No problems with lateral shift in alignment

Modular design - multiple configurations available - this one has all the .
Simplified sample preparation - no washes, no dissolution options, no clogging fluids.
Enable unique application-complex protocols on a wide range of cell types and samples.
Customized 4 laser, 14 color system.
Data up to 35,000 events per second.
Particles from 0.5 μm to 50 μm in diameter can be detected.

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