Kingella Kingae Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0650-ZJF)

Highlights

Easy-start kit: DNA template is the only requirement.
Conserved-region targeting ensures reliable specificity.
Optimized formulation supports high-sensitivity detection.

Cat Number: STEM-SLSR-0650-ZJF

Application: For qualitative and quantitative analysis of Kingella Kingae

Model: 24593




Description

Utilizing SYBR Green real-time PCR technology, this kit is engineered for the detection and quantification of Kingella Kingae. Specific primers ensure selective amplification of the target gene. As fluorescence increases with amplification, the system provides precise and reproducible data for both qualitative and quantitative purposes.

Specification

Kit Components:
Kingella Kingae Amplification Mix: 50 µL × 1 tube
Kingella Kingae Reaction Mix: 950 µL × 1 tube
Kingella Kingae Positive Control: 50 µL × 1 tube
Kingella Kingae Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Features

Easy-start kit: DNA template is the only requirement.
Conserved-region targeting ensures reliable specificity.
Optimized formulation supports high-sensitivity detection.
Positive sample control included to validate results.
Self-contained PCR setup prevents external contamination.
Restricted to experimental research usage.

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