Investigatation of lipid peroxidation caused by post-ischemic reperfusion by Electron paramagnetic resonance (EPR) spectroscopy (CAT#: STEM-MB-1018-WXH)

Name: Investigatation of lipid peroxidation caused by post-ischemic reperfusion by Electron paramagnetic resonance (EPR) spectroscopy
SKU: STEM-MB-1018-WXH
Rating: 5 (1 reviews)

Introduction

Lipid peroxidation can be described generally as a process under which oxidants such as free radicals attack lipids containing carbon-carbon double bond(s), especially polyunsaturated fatty acids (PUFAs). Lipid peroxidation is a metabolic process that causes oxidative deterioration of lipids by reactive oxygen species. This process can degrade the lipids within the cell membrane leading to cell damage and eventually, cell death. Oxidative deterioration is caused by highly reactive free radical species.

Buy Now

Principle Applications Procedure Materials

Principle

Electron Paramagnetic Resonance (EPR), also called Electron Spin Resonance (ESR), is a branch of magnetic resonance spectroscopy which utilizes microwave radiation to probe species with unpaired electrons, such as radicals, radical cations, and triplets in the presence of an externally applied static magnetic field.
EPR spectroscopy is particularly suitable for the investigation of (bio)chemical systems with strongly localized spin density and their interaction with the environment. For these systems EPR provides information on the structure and dynamics and is widely used in chemistry, physics and biology.

Applications

• Study dynamic organisation of lipids in biological membranes, lipid-protein interactions and temperature of transition of gel to liquid crystalline phases.
• Determine oxygen levels in tissues and blood.
• Injection of spin-labeled molecules allows for electron resonance imaging of living organisms.
• EPR can be used to measure microviscosity and micropolarity within drug delivery systems as well as the characterization of colloidal drug carriers.
• The study of radiation-induced free radicals in biological substances (for cancer research).
• Investigation on the antioxidant properties of medicine