Immobilized Iminobiotin, 5ml Resin, 786-599, G-BIOSCIENCES (CAT#: STEM-C-3119-LGZ)

The immobilized imine biotin resin consists of imine biotin, a cyclic guanine analog of biotin, covalently coupled to 6% cross-linked agarose. This resin allows the purification of avidin, streptavidin and neutravidin with their subsequent gentle elution using non-denaturing elution buffer.

The normal biotin-avidin complex requires a strong denaturing buffer, 8M guanidine·HCl, to denature avidin and release biotin, which obviously destroys the native and functional aspects of avidin. The iminobiotin-avidin complex is formed at pH 9.5 and can be separated at pH 4.0 with a mild elution buffer consisting of 50 mM ammonium acetate, pH 4.0, 0.5M NaCl.

Size: 5ml resin

Biotin Binding Capacity: >2mg avidin/ml resin
No requirement for strong, denaturing elution buffers
Elutes at pH4.0

Co-NTA Spin Plates, 2 Plates, 786-988, G-BIOSCIENCES (CAT#-STEM-C-3158-LGZ)
Cobalt Chelating Resin, High Capacity >50mg/ml, 10ml Resin, 786-286, G-BIOSCIENCES (CAT#-STEM-C-3159-LGZ)
Cobalt Chelating Resin, High Capacity >50mg/ml, 100ml Resin, 786-402, G-BIOSCIENCES (CAT#-STEM-C-3160-LGZ)
Cobalt Chelating Resin, High Capacity >50mg/ml, 500ml Resin, 786-403, G-BIOSCIENCES (CAT#-STEM-C-3161-LGZ)
Cobalt Chelating Resin, 0.2ml Spin Column, High Capacity >50mg/ml, 25 Columns, 786-454, G-BIOSCIENCES (CAT#-STEM-C-3162-LGZ)
Cobalt Chelating Resin, 1ml Spin Column, High Capacity >50mg/ml, 5 Columns, 786-455, G-BIOSCIENCES (CAT#-STEM-C-3163-LGZ)