Identification of living spermatogenic cells of the mouse for 'in situ' analyses of DNA polymerase activities by transillumination-phase contrast microscopic technique (CAT#: STEM-MIT-0305-LJX)

Phase contrast microscope is a microscope that uses the difference of refractive index and thickness between different structural components of an object to change the optical path difference through different parts of the object into the difference of amplitude (light intensity), and realizes observation through a condenser with a ring diaphragm and a phase objective.
Phase difference refers to the difference in phase caused by the same light passing through a medium with different refractive indices. Phase refers to the position reached by the fluctuation of light at a certain time. Generally, due to the small difference that the object being tested can produce, phase difference is difficult for the naked eye to distinguish, and it can only be distinguished after changing the difference to amplitude difference (brightness difference). The difference is determined by the difference in refractive index and thickness of the medium through which light waves pass, which is equal to the difference in refractive index multiplied by thickness (i.e. the difference in optical path). The phase contrast microscope uses the difference in optical path of the object being tested for microscopic examination.

For observation of living cells, non-stained tissue sections and the lack of contrast stained samples

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

• Sample Type:
Living spermatogenic cells of the mouse

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures