Hot Start PCR (CAT#: STEM-MB-0190-WXH)

Hot start PCR follows the same principles as the conventional PCR - in that it uses DNA polymerase to synthesise DNA from a single stranded template. However, it utilizes additional heating and separation methods, such as inactivating or inhibiting the binding of Taq polymerase and late addition of Taq polymerase, to increase product yield as well as provide a higher specificity and sensitivity. Non-specific binding and priming or formation of primer dimers are minimized by completing the reaction mix after .

• Reduces non-specific amplification.
• Offers the convenience of reaction set up at room temperature.
• Improve specificity and sensitivity.
• Increase the product yield of the targeted fragment.

Methods:
(1)Inactivation/inhibition of Taq DNA polymerase
(2)Late addition of Taq DNA polymerase
(3)Deoxyribonucleotide triphosphate (dNTP) modifications
(4)Modified primers
(5)Controlled addition of magnesium