Glutathione Resin Spin Plates, 2 Plates, 786-993, G-BIOSCIENCES (CAT#: STEM-C-3150-LGZ)

Glutathione resins are designed for affinity purification of proteins with glutathione s-transferase (GST) tagging. The resin consists of reduced glutathione (GSH) covalently coupled to 6% cross-linked agarose via a 10-carbon spacer arm. The resin has a binding capacity of 40 mg GST/ml resin. Supplied as a 20% ethanol slurry.

Resins, columns and binding/wash and elution buffers are available separately.

The resin bed volumes of the spin columns were 0.2, 1 and 3 ml, respectively, and the total column volumes were 1, 8 and 22 ml, respectively. The column is available as a spin format for gravity flow columns.

Can also process up to 96 samples in a 96-well spin plate format.

Our new HOOK™ GST Protein Purification Kit is available now and includes everything needed to purify GST-tagged proteins from yeast or bacteria, including lysis buffer, lysing enzyme, resin, column and binding, washing and elution buffers. Available kits are:

HOOK™ GST Protein Purification (Bacteria): Isolation from bacteria; optimized to produce up to 10mg/250ml of soluble GST-tagged protein.
HOOK™ GST Protein Spin Purification (Bacteria): Isolation from bacteria; culture optimized for production of ~1mg/50ml soluble GST-tagged protein
HOOK™ GST Protein Purification (Yeast): Isolated from yeast; optimized to yield up to 10 mg of soluble GST-tagged protein.
HOOK™ GST Protein Spin Purification (Yeast): Isolated from yeast; optimized to yield up to 1 mg of soluble GST-tagged protein.
Purified GST protein can also be used as a positive control for purification and Western blotting.

Size: 2 plates

For the purification of GST proteins
High capacity (>40mg/ml)
Bead size: 50-160μm
Bead structure: 6% cross-linked agarose
10 carbon spacer arm.

Amine Magnetic Beads, ~50mM Ligand density, 5ml Resin, 786-907, G-BIOSCIENCES (CAT#-STEM-C-3110-LGZ)
Carbohydrate Immobilization Kit, For 5 x 2ml Column, 786-807, G-BIOSCIENCES (CAT#-STEM-C-3111-LGZ)
Carbohydrate Coupling Resin, 10ml Resin, 786-808, G-BIOSCIENCES (CAT#-STEM-C-3112-LGZ)
HOOK™ Activated Agarose (Amine Reactive), 10ml Resin, 786-066, G-BIOSCIENCES (CAT#-STEM-C-3113-LGZ)
Immobilized O-Phosphorylethanolamine, 5 ml, 6% Cross-Linked Agarose, 786-1310, G-BIOSCIENCES (CAT#-STEM-C-3114-LGZ)
Sulfhydryl Coupling Resin, 10ml Resin, 786-794, G-BIOSCIENCES (CAT#-STEM-C-3115-LGZ)