G-Trap™ Ion Exchange Selection Kit, 4 x 1ml Columns, 786-1018, G-BIOSCIENCES (CAT#: STEM-C-3311-LGZ)

G-Trap™ Ion Exchange Selection Kit contains 4 different types of 1ml G-Trap™ Ion Exchange Agarose Fast Flow Columns for initial screening of suitable ion exchange resins and/or pH or ionic strength optimization for priming and elution Buffer, separation of target protein or substance by ion exchange chromatography.

Biomolecules or proteins carrying a net charge opposite to the ion exchanger bind to the ion exchanger and thereby separate them from the bulk molecules in the sample.

The G-Trap™ Ion Exchange Selection Kit contains each, G-Trap™ CM Agarose Express, G-Trap™ SP Agarose Express, G-Trap™ DEAE Agarose Express, and G-Trap™ Q Agarose Fast flow column.

After preliminary screening of suitable ion exchange resins by G-Trap™ Ion Exchange Selection Kit, separate G-Trap™ Ion Exchange Sepharose Fast Flow Columns can be ordered in bulk as needed.

G-Trap™ Ion Exchange Agarose Fast Flow Columns are available in 1ml and 5ml column volumes namely G-Trap™ CM Agarose Fast Flow, G-Trap™ SP Agarose Fast Flow, G-Trap™ DEAE Agarose Fast Flow and G-Trap™ Q Sepharose Fast Flow Columns.

G-Trap™ Ion Exchange Agarose Fast Flow Columns can separate biomolecules on the basis of charge using prepacked columns. G-Trap™ Ion Exchange Agarose Fast Flow Columns are used for method detection, separation or purification of biomolecules, sample concentration and sample cleanup of charged molecules.

Size: 4 x 1ml columns

G-Trap™ CM Agarose Fast Flow resin

Ion Exchanger: Weak cation exchanger
Matrix: Cross-linked agarose beads, 6%
Bead size: 45-165 µm
Ligand: Carboxymethyl
Ionic Capacity: 0.09-0.13 mmol (H+)/ml
Binding Capacity: 50 mg lysozyme/ ml medium
pH stability Working Range: 4-12
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Flow Velocity: 450cm/h
Recommended Flow rate: 1 ml/ min and 5 ml/ min for 1 ml and 5 ml column respectively
Maximum Pressure: 0.3MPa
Exclusion limit(globular proteins): 4 x 106
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8M urea, 8M guanidine hydrochloride, 70% ethanol
Storage Conditions: 4 to 30°C, 20% Ethanol

G-Trap™ SP Agarose Fast Flow resin

Ion Exchanger: Strong cation exchanger
Matrix: Cross-linked agarose beads, 6%
Bead size: 45-165 µm
Ligand: Sulphopropyl
Ionic Capacity: 0.18-0.25 mmol (Na+)/ml
Binding Capacity: 70 mg lysozyme /ml medium
pH stability Working Range: 2-12
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Flow Velocity: 450cm/h
Recommended Flow rate: 1 ml/ min and 5 ml/ min for 1 ml and 5 ml column respectively
Maximum Pressure: 0.3MPa
Exclusion limit(globular proteins): 4 x 106
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8M urea, 8M guanidine hydrochloride, 70% ethanol
Storage Conditions: 4 to 30°C, 20% Ethanol containing 0.2M sodium acetate

G-Trap™ DEAE Agarose Fast Flow resin

Ion Exchanger: Weak anion exchanger
Matrix: Cross-linked agarose beads, 6%
Bead size: 45-165 µm
Ligand: Diethylaminoethyl
Ionic Capacity: 0.11-0.16mmol (Cl-)/ml
Binding Capacity: 90mg HSA/ml medium
pH stability Working Range: 2-9
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Flow Velocity: 450cm/h
Recommended Flow rate: 1 ml/ min and 5 ml/ min for 1 ml and 5 ml column respectively
Maximum Pressure: 0.3MPa
Exclusion limit(globular proteins): 4 x 106
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8M urea, 8M guanidine hydrochloride, 70% ethanol
Storage Conditions: 4 to 30°C, 20% Ethanol

G-Trap™ Q Agarose Fast Flow resin

Ion Exchanger: Strong anion exchanger
Matrix: Cross-linked agarose beads, 6%
Bead size: 45-165 µm
Ligand: Quaternary ammonium
Ionic Capacity: 0.18-0.25mmol (Cl-)/ml
Binding Capacity: 120 mg HSA /ml medium
pH stability Working Range: 2-12
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Flow Velocity: 450cm/h
Recommended Flow rate: 1 ml/ min and 5 ml/ min for 1 ml and 5 ml column respectively
Maximum Pressure: 0.3MPa
Exclusion limit(globular proteins): 4 x 106
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8M urea, 8M guanidine hydrochloride, 70% ethanol
Storage Conditions: 4 to 30°C, 20% Ethanol

G-Trap™ Ion Exchange Selection Kit, 1 ml columns

Column Volume: 1 ml
Columns Dimension: 0.7 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)

Spin Column, 1ml, 25 Columns, 786-810, G-BIOSCIENCES (CAT#-STEM-C-3331-LGZ)
Spin Column, 1ml, 50 Columns, 786-811, G-BIOSCIENCES (CAT#-STEM-C-3332-LGZ)
Twist Break Column, 25 Columns, 786-1810, G-BIOSCIENCES (CAT#-STEM-C-3333-LGZ)
Column, 20ml, 25 Columns, 786-197, G-BIOSCIENCES (CAT#-STEM-C-3334-LGZ)
Columns, 5ml, 25 Columns, 786-169, G-BIOSCIENCES (CAT#-STEM-C-3335-LGZ)
G-Trap™ 1ml FliQ FPLC Column, 1 Column, 786-1292, G-BIOSCIENCES (CAT#-STEM-C-3336-LGZ)