G-Trap™ HIC Selection Kit, 4 x 1ml Columns, 786-1009, G-BIOSCIENCES (CAT#: STEM-C-3292-LGZ)

G-Trap™ HIC Selection Kit consists of 4 pre-packed 1ml columns (G-Trap™ HIC Columns) with matrix or resin for hydrophobic interaction chromatography. The kit consists of 4 packing columns, namely G-Trap™ Butyl Sepharose 6 Fast Flow, G-Trap™ Octyl Sepharose 6 Fast Flow, G-Trap™ Phenyl Sepharose 6 Fast Flow (High Times) and G-Trap™ Phenyl Sepharose 6 fast flow (low order).

The G-Trap™ HIC Selection Kit allows initial screening for appropriate ligands/substrates and conditions for the isolation of specific proteins or biomolecules. Once a suitable resin/media has been identified, G-Trap™ HIC columns can be used in 1ml or 5ml sizes. Individual G-Trap™ HIC columns are available at G-biosciences in 1 ml and 5 ml column volume sizes.

G-Trap™ HIC columns can be used to separate target proteins, including G-Trap™ Butyl Sepharose 6 Fast Flow, G-Trap™ Octyl Sepharose 6 Fast Flow, G-Trap™ Phenyl Sepharose 6 Fast Flow ( High flow) and G-Trap™ Phenyl Sepharose 6 Fast Flow (low flow).

Biomolecules are separated on HIC columns according to the hydrophobicity of the ligands attached to the resin, the distribution of surface-exposed hydrophobic amino acids on the protein or enzyme to be separated, and the concentration and type of salt used for binding or priming buffers.

Straight alkyl chain ligands (such as butyl, octyl) exhibit purely hydrophobic properties, aryl ligands (such as phenyl) exhibit mixed modes, and aromatic, hydrophobic, and uncharged play a role on protein/biomolecule adsorption columns. effect. In HIC, the binding of biomolecules to the column (ligand) is facilitated by using a high concentration of anti-orientation salts (such as ammonium sulfate, sodium sulfate, etc.) in the binding buffer.

Size: 4 x 1ml columns

G-Trap™ Butyl Agarose 6 Fast Flow Resin

Mean particle size: 90 µm
Bead structure: Highly cross-linked 6% Agarose
Ligand: Butyl
Ligand Concentration: About 40μmol/ml
Binding Capacity: About 20mg HSA/ml resin
pH stability Working Range: 3-13
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Pressure (MPa): 0.3
Maximum Flow Velocity: 450cm/h
Exclusion Limit (Globular Proteins): 4 x 106
Physical Stability: Negligible volume variation due to changes in pH or ionic strength
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol
Storage Conditions: 4 to 30°C, 20% Ethanol

G-Trap™ Octyl Agarose 6 Fast Flow Resin

Mean particle size: 90 µm
Bead structure: Highly cross-linked 6% Agarose
Ligand: Octyl
Ligand Concentration: About 5μmol/ml
Binding Capacity: About 30mg HSA/ml resin
pH stability Working Range: 3-13
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Pressure (MPa): 0.3
Maximum Flow Velocity: 450cm/h
Exclusion Limit (Globular Proteins): 4 x 106
Physical Stability: Negligible volume variation due to changes in pH or ionic strength
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol
Storage Conditions: 4 to 30°C, 20% Ethanol

G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub) Resin

Mean particle size: 90 µm
Bead structure: Highly cross-linked 6% Agarose
Ligand: Phenyl
Ligand Concentration: About 40μmol/ml
Binding Capacity: About 40mg HSA/ml resin
pH stability Working Range: 3-13
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Pressure (MPa): 0.3
Maximum Flow Velocity: 450cm/h
Exclusion Limit (Globular Proteins): 4 x 106
Physical Stability: Negligible volume variation due to changes in pH or ionic strength
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol
Storage Conditions: 4 to 30°C, 20% Ethanol

G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub) Resin

Mean particle size: 90 µm
Bead structure: Highly cross-linked 6% Agarose
Ligand: Phenyl
Ligand Concentration: About 25 μmol/ml
Binding Capacity: About 20 mg HSA/ml resin
pH stability Working Range: 3-13
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Pressure (MPa): 0.3
Maximum Flow Velocity: 450 cm/h
Exclusion Limit (Globular Proteins): 4 x 106
Physical Stability: Negligible volume variation due to changes in pH or ionic strength
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol
Storage Conditions: 4 to 30°C, 20% Ethanol

G-Trap™ HIC Selection Kit, 1 ml column

Column Volume: 1 ml
Columns Dimension: 0.7 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)

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