Fifth Fever Bartonella Probe-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-2447-ZJF)

Employing probe-based real-time PCR, this kit detects Fifth Fever Bartonella by amplifying its conserved genetic regions with highly specific primers. Fluorescence signals are emitted during amplification and continuously monitored, allowing for accurate qualitative and quantitative analysis of Fifth Fever Bartonella nucleic acids.

Kit Components:
Fifth Fever Bartonella Amplification Mix: 50 µL × 1 tube
Fifth Fever Bartonella Reaction Mix: 950 µL × 1 tube
Fifth Fever Bartonella Positive Control: 50 µL × 1 tube
Fifth Fever Bartonella Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: Reagents in the kit are sufficient for the number of tests indicated on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light, avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
Compatible with real-time PCR systems including ABI, MX3000P/3005P, LightCycler, Bio-Rad, and Eppendorf.
Additional required equipment: DNase/RNase-free 1.5 mL centrifuge tubes, 0.2 mL PCR reaction tubes, filter tips (10 µL, 200 µL, 1000 µL), centrifuge, vortex mixer, and pipettes of various capacities.
Sample Requirements:
Depending on circumstances, use intestinal tissue from dead or culled cattle under sterile conditions. For live animals, samples may include whole blood, serum, plasma, feces, vomit, oral/nasopharyngeal/respiratory secretions, throat/nasal/ocular/anal swabs, or viral cultures.
Cross-contamination must be avoided. Samples should be tested promptly after collection or stored at -20°C ± 5°C. For long-term storage, samples should be kept below -70°C.
Sample Storage and Transport:
Short-term: Store at -20°C.
Long-term (up to 6 months): Store below -70°C.
Transport using 2-8°C ice packs. Repeated freeze-thaw cycles are strictly prohibited.

User-friendly design with ready-to-go reagent mix.
Conserved-region primer/probe sets ensure accurate targeting.
Sensitivity enhanced through meticulous component tuning.
Supplied with a control sample to confirm test results.
Fully enclosed PCR system lowers contamination probability.
Rapid amplification process shortens test duration.
Suitable only for non-clinical laboratory use.

STEM-SLSR-2445-ZJF
STEM-SLSR-2446-ZJF
STEM-SLSR-2448-ZJF
STEM-SLSR-2449-ZJF