Enumeration and phenotyping of circulating microvesicles by Nanoparticle tracking analysis (NTA) (CAT#: STEM-MB-0719-WXH)

Name: Enumeration and phenotyping of circulating microvesicles by Nanoparticle tracking analysis (NTA)
SKU: STEM-MB-0719-WXH
Rating: 5 (1 reviews)

Introduction

Microvesicles (MVs) are bioactive, submicron-sized (0.01-1000 nm) membrane vesicles released from various types of cells under normal physiological and pathophysiological conditions. MVs have emerged as important mediators of cell-to-cell communication in a diverse range of normal and pathological processes. MVs have been recognized as potential biomarkers in coagulation, inflammation, and cancer. However, for clinical use, minimizing factors which could affect enumeration and phenotypic characterization of MVs during pre-analytical steps is crucial.

Buy Now

Principle Applications Procedure Materials

Principle

Nanoparticle tracking analysis (NTA) is a method for visualizing and analyzing particles in liquids that relates the rate of Brownian motion to particle size. The rate of movement is related only to the viscosity and temperature of the liquid; it is not influenced by particle density or refractive index. NTA allows the determination of a size distribution profile of small particles with a diameter of approximately 10-1000 nanometers (nm) in liquid suspension.

Applications

NTA has been used by commercial, academic, and government laboratories working with nanoparticle toxicology, drug delivery, exosomes, microvesicles, bacterial membrane vesicles, and other small biological particles, virology and vaccine production, ecotoxicology, protein aggregation, orthopedic implants, inks and pigments, and nanobubbles.