Enterohemorrhagic Escherichia Coli (EHEC) Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0853-ZJF)

This real-time PCR detection kit is based on SYBR Green fluorescence chemistry and is intended for the analysis of Enterohemorrhagic Escherichia Coli (EHEC) nucleic acids. Primers targeting conserved regions enable specific amplification. During the reaction, fluorescent signals are emitted and continuously tracked by the system, providing reliable, real-time quantification and identification of the target sequence.

Kit Components:
Enterohemorrhagic Escherichia Coli (EHEC) Amplification Mix: 50 µL × 1 tube
Enterohemorrhagic Escherichia Coli (EHEC) Reaction Mix: 950 µL × 1 tube
Enterohemorrhagic Escherichia Coli (EHEC) Positive Control: 50 µL × 1 tube
Enterohemorrhagic Escherichia Coli (EHEC) Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Simplified protocol: only DNA needs to be added.
High-precision primers designed for analyte-specific detection.
Reagents optimized for robust signal sensitivity.
Positive control supports result validation and QC.
Fully enclosed PCR setup mitigates contamination risks.
Limited to academic or laboratory research.

STEM-SLSR-0851-ZJF
STEM-SLSR-0852-ZJF
STEM-SLSR-0854-ZJF
STEM-SLSR-0855-ZJF