Distinguishing between Phosphorylated and Nonphosphorylated Peptides by Ion Mobility−Mass Spectrometry (CAT#: STEM-ST-0148-LJX)

Introduction

Mass spectrometry has become an indispensable tool in identifying post-translationally modified proteins, but multiple peptide mass-mapping/peptide-sequencing experiments are required to answer questions involving the site and type of modification present. Here, we apply ion mobility−mass spectrometry (IM−MS), a high-throughput analysis method having high selectivity and sensitivity, to the challenge of identifying phosphorylated peptides. Ion mobility separation is based on the collision cross-section of the ion. Phosphorylation can result in a conformational change in gas-phase peptide ions, which can be detected by IM.