Diarrheagenic Escherichia coli (Diarrheagenic E. coli) Universal Probe-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-2237-ZJF)

Using high-performance probes and precision-designed primers, this real-time PCR kit enables the detection of Diarrheagenic Escherichia coli (Diarrheagenic E. coli) Universal by targeting conserved genomic regions. Fluorescence signals are emitted as the target is amplified, and the system records data in real time for confident qualitative and quantitative evaluation.

Kit Components:
Diarrheagenic Escherichia coli (Diarrheagenic E. coli) Universal Amplification Mix: 50 µL × 1 tube
Diarrheagenic Escherichia coli (Diarrheagenic E. coli) Universal Reaction Mix: 950 µL × 1 tube
Diarrheagenic Escherichia coli (Diarrheagenic E. coli) Universal Positive Control: 50 µL × 1 tube
Diarrheagenic Escherichia coli (Diarrheagenic E. coli) Universal Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: Reagents in the kit are sufficient for the number of tests indicated on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light, avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
Compatible with real-time PCR systems including ABI, MX3000P/3005P, LightCycler, Bio-Rad, and Eppendorf.
Additional required equipment: DNase/RNase-free 1.5 mL centrifuge tubes, 0.2 mL PCR reaction tubes, filter tips (10 µL, 200 µL, 1000 µL), centrifuge, vortex mixer, and pipettes of various capacities.
Sample Requirements:
Depending on circumstances, use intestinal tissue from dead or culled cattle under sterile conditions. For live animals, samples may include whole blood, serum, plasma, feces, vomit, oral/nasopharyngeal/respiratory secretions, throat/nasal/ocular/anal swabs, or viral cultures.
Cross-contamination must be avoided. Samples should be tested promptly after collection or stored at -20°C ± 5°C. For long-term storage, samples should be kept below -70°C.
Sample Storage and Transport:
Short-term: Store at -20°C.
Long-term (up to 6 months): Store below -70°C.
Transport using 2-8°C ice packs. Repeated freeze-thaw cycles are strictly prohibited.

User-friendly design with ready-to-go reagent mix.
Conserved-region primer/probe sets ensure accurate targeting.
Sensitivity enhanced through meticulous component tuning.
Supplied with a control sample to confirm test results.
Fully enclosed PCR system lowers contamination probability.
Rapid amplification process shortens test duration.
Suitable only for non-clinical laboratory use.

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