Determination of Nucleic Acids with Crystal Violet by a Resonance Light-Scattering Technique (CAT#: STEM-ST-0037-YJL)

Name: Determination of Nucleic Acids with Crystal Violet by a Resonance Light-Scattering Technique
SKU: STEM-ST-0037-YJL
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Introduction

The quantitative analysis of nucleic acids is of great interest since it is often used as a reference for measurements of other components in biological samples. Up to now, many techniques such as spectrophotometric methods, radioactive labeling and fluorimetric methods have been developed to determine nucleic acids.
Crystal violet (CV) is a cationic triphenyl methane dye chemically known as hexamethyl pararosaniline chloride. It is a highly demanded industrial raw material used in far-reaching industrial and medical applications.

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Principle Applications Procedure Materials

Principle

Resonance Rayleigh scattering (RRS) is similar to Rayleigh scattering in nature. Resonance Rayleigh scattering is a special elastic scattering produced when the wavelength of Rayleigh scattering (RS) is located at or close to its molecular absorption band. The key to generating RRS is: when the scattering is at or close to the absorption band of the scattering molecule, since the electron absorbs the electromagnetic wave at the same frequency as the scattering frequency, the electron strongly absorbs the photon energy due to resonance and re-scatters. Its scattering intensity is several orders of magnitude higher than that of pure Rayleigh scattering, and it no longer obeys the Rayleigh law of I∝λ-4. This absorption-rescattering process is called resonance Rayleigh scattering (RRS).

Applications

Resonance Rayleigh scattering is used to the study of aggregation of chromophores on biological macromolecules and the determination of biological macromolecules such as nucleic acid, proteins and heparin, further, it has been used in the determination of trace amounts of inorganic ions and the cationic surfactant by means of ion association reactions with some dyes. In addition, it has been applied to the study of nanoparticles in liquid and the determination of β-cyclodextrin inclusion constant and the critical micelle concentration of surfactant.