Determination of Nucleic Acids Based on Their Resonance Light Scattering Enhancement Effect on Metalloporphyrin Derivatives (CAT#: STEM-ST-0038-YJL)

Introduction

The assay of nucleic acids is of great importance in the fields of analytical biochemistry and clinical medicine. Many techniques, including spectrophotometry and fluorimetry, have been used to determine nucleic acids. Traditional ultraviolet spectrophotometry based on absorption at 260 nm is simple, but it suffers from low sensitivity and serious interferences. <br />Tetraphenyl porphyrin cobalt chlorine (CoTPPCl), tetraphenyl porphyrinatoiron chlorine (FeTPPCl), and m-oxotetraphenyl porphyrinatoiron [(FeTPP)2O] are all metalloporphyrin derivatives. They were used as catalyzers and neutral carriers for PVC membrane electrodes.

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Principle Applications Procedure Materials Notes Related Products

Principle

Resonance Rayleigh scattering (RRS) is similar to Rayleigh scattering in nature. Resonance Rayleigh scattering is a special elastic scattering produced when the wavelength of Rayleigh scattering (RS) is located at or close to its molecular absorption band. The key to generating RRS is: when the scattering is at or close to the absorption band of the scattering molecule, since the electron absorbs the electromagnetic wave at the same frequency as the scattering frequency, the electron strongly absorbs the photon energy due to resonance and re-scatters. Its scattering intensity is several orders of magnitude higher than that of pure Rayleigh scattering, and it no longer obeys the Rayleigh law of I∝λ-4. This absorption-rescattering process is called resonance Rayleigh scattering (RRS).

Applications

Resonance Rayleigh scattering is used to the study of aggregation of chromophores on biological macromolecules and the determination of biological macromolecules such as nucleic acid, proteins and heparin, further, it has been used in the determination of trace amounts of inorganic ions and the cationic surfactant by means of ion association reactions with some dyes. In addition, it has been applied to the study of nanoparticles in liquid and the determination of β-cyclodextrin inclusion constant and the critical micelle concentration of surfactant.

Procedure

1. Sample preparation
2. Measurement by scattering detection instrument
3. Data analysis

Materials

Rayleigh scattering measurement system

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