Detection of dynamin crystallization processes by Dual polarization interferometry (DPI) (CAT#: STEM-MB-0392-WXH)

Name: Detection of dynamin crystallization processes by Dual polarization interferometry (DPI)
SKU: STEM-MB-0392-WXH
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Introduction

Dynamin is a protein required for vesicle formation during synaptic vesicle endocytosis (SVE) and for clathrin-mediated endocytosis. Dynamin is a GTPase that plays a vital role in clathrin-dependent endocytosis and other vesicular trafficking processes by acting as a pair of molecular scissors for newly formed vesicles originating from the plasma membrane.

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Principle Applications Procedure Materials

Principle

Dual polarization interferometry (DPI) is an analytical technique that allows the simultaneous determination of thickness, density, and mass of a biological layer on a sensing waveguide surface in real time. DPI focuses laser light into two waveguides. One of these functions as the "sensing" waveguide having an exposed surface while the second one functions to maintain a reference beam. A two-dimensional interference pattern is formed in the far field by combining the light passing through the two waveguides. The DPI technique rotates the polarization of the laser, to alternately excite two polarization modes of the waveguides. Measurement of the interferogram for both polarizations allows both the refractive index and the thickness of the adsorbed layer to be calculated. These measurements can be used to infer conformational information about the molecular interactions taking place, as the molecule size (from the layer thickness) and the fold density (from the RI) change.

Applications

Detection of dynamin crystallization processes.
Drug discovery.
Study the molecular structure of the dynamin.

Procedure

1. Setting of dual polarization interferometry
2. Preparing the DPI sensor chip
3. Immobilization of target on DPI biosensor
4. Reagent was injected to react
5. Quantitative analysis