DEAE Seplife FF Weak Anion-Exchange Medium, 750 cm/h (CAT#: STEM-BC-3165-ZJF)

This weak anion-exchange medium is designed for downstream purification of proteins, nucleic acids, and peptides. It uses highly crosslinked high-flow agarose functionalized with diethylaminoethyl groups. The weak anion-exchange ligand provides controllable pH-dependent selectivity. The 45-165 µm particles provide high permeability and relatively low backpressure. A BSA adsorption capacity above 50 mg/mL supports preparative capture and intermediate purification. The medium provides a maximum stated flow rate of 750 cm/h. Broad chemical compatibility supports use with common buffers, denaturants, ethanol, and sodium hydroxide. The matrix tolerates steam treatment and can be scaled for manufacturing applications.

Model: DEAE Seplife FF
Appearance: White spherical gel, odorless and tasteless
Type: Weak anion-exchange medium
Matrix: Seplife 6FF
Ligand: Diethylaminoethyl
Particle size: 45-165 µm
BSA adsorption capacity:>50 mg/mL
Ion-exchange capacity: 0.11-0.16 mmol/mL
Maximum flow rate: 750 cm/h
Pressure resistance: 0.3 MPa
Operating temperature: 4-40°C
Long-term pH stability: 2-12
Short-term CIP pH stability: 1-14
Chemical compatibility: Common aqueous buffers, 1 mol/L sodium hydroxide, 8 mol/L urea, 8 mol/L guanidine hydrochloride, and 70% ethanol
Heat resistance: 121°C in water for 30 min

Weak anion-exchange selectivity
Up to 750 cm/h flow
>50 mg/mL BSA capacity
Low operating backpressure
Broad chemical stability
Wide CIP pH tolerance
Steam-resistant matrix
Scalable process performance

STEM-BC-0412-ZJF
STEM-BC-3074-ZJF
STEM-BC-3075-ZJF
STEM-BC-3078-ZJF
STEM-BC-3081-ZJF