DEAE Seplife BB Weak Anion-Exchange Medium, 1200-1800 cm/h (CAT#: STEM-BC-3166-ZJF)

This weak anion-exchange medium is designed for purification of proteins, nucleic acids, and peptides. It uses six-percent crosslinked agarose functionalized with diethylaminoethyl groups. The weak-base ligand provides pH-dependent anion-exchange selectivity. Large spherical particles create a highly permeable packed bed with low backpressure. The medium supports flow from 1200 to 1800 cm/h under the specified differential pressure. Broad chemical stability permits use with common buffers, sodium hydroxide, denaturants, and ethanol. The agarose matrix tolerates the stated steam-treatment conditions. Its high-flow format is suitable for rapid large-scale capture and intermediate purification.

Model: DEAE Seplife BB
Appearance: White spherical gel, odorless and tasteless
Type: Weak anion-exchange medium
Matrix: 6% crosslinked agarose
Ligand: Diethylaminoethyl
Median particle size d50: 200 µm
Particle-size range: 100-300 µm
Ion-exchange capacity: 0.11-0.16 mmol/mL
Flow range: 1200-1800 cm/h at 0.1 MPa differential pressure and 25 cm bed height
Operating temperature: 4-40°C
Long-term pH stability: 2-12
Short-term CIP pH stability: 1-14
Chemical compatibility: Common aqueous buffers, 1 mol/L sodium hydroxide, 8 mol/L urea, 8 mol/L guanidine hydrochloride, and 70% ethanol
Heat resistance: 121°C in water for 30 min
Storage: 20% ethanol at 4-30°C

Ultra-high linear flow
Large low-backpressure particles
Weak DEAE selectivity
Broad CIP pH range
Strong chemical compatibility
Steam-resistant matrix
Suitable for rapid capture
Scalable large-column operation

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