DEAE Seplife A25 Weak Anion-Exchange Medium, 140 mg α-Lactalbumin/mL (CAT#: STEM-BC-3173-ZJF)

This weak anion-exchange medium is designed for separation of low-molecular-weight proteins, peptides, nucleotides, and selected large molecules. It uses highly crosslinked dextran G25 as the matrix. Diethylaminoethyl groups provide weak anion-exchange functionality. The dry particles range from 40 to 120 µm. An α-lactalbumin adsorption capacity of 140 mg/mL supports high-capacity purification. The medium provides high flow with relatively low backpressure. Its ion-exchange capacity supports preparative biomolecule processing. The material is stable in common aqueous buffers and concentrated denaturants. The matrix also tolerates the stated steam-treatment conditions.

Model: DEAE Seplife A25
Appearance: White particles, odorless and tasteless
Type: Weak anion-exchange medium
Matrix: Dextran G25
Ligand: Diethylaminoethyl
Dry particle size: 40-120 µm
α-Lactalbumin adsorption capacity: 140 mg/mL
Ion-exchange capacity: 3.0-4.0 mmol/g dry gel
Working pH range: 2-9
Long-term pH stability: 2-13
Short-term pH stability: 2-13
Chemical compatibility: Common aqueous buffers, 8 M urea, and 6 M guanidine hydrochloride
Heat resistance: 121°C in 0.1 M sodium chloride for 30 min
Stated separation application: Molecules including proteins, peptides, nucleotides, and species above 30000 molecular weight

High 140 mg/mL capacity
Weak DEAE exchange
Crosslinked dextran G25
Broad pH stability
Denaturant-compatible operation
Steam-treatment tolerance
Suitable for diverse molecular sizes
Scalable preparative processing

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