DEAE Large Scale HP Weak Anion-Exchange Medium, 90 mg Ovalbumin/mL (CAT#: STEM-BC-3160-ZJF)

This weak anion-exchange medium is designed for purification of proteins, nucleic acids, and peptides. It uses small high-flow agarose microspheres functionalized with diethylaminoethyl groups. The approximately 36-44 µm particles provide efficient mass transfer and improved resolution. A stated ovalbumin adsorption capacity of 90 mg/mL supports productive preparative purification. Weak anion-exchange chemistry provides process selectivity different from strong quaternary-ammonium media. The medium operates at 220 cm/h with low backpressure under the stated conditions. Low nonspecific adsorption supports high product recovery. Broad chemical compatibility permits repeated cleaning with sodium hydroxide and exposure to denaturants and ethanol. The matrix also tolerates the specified steam-treatment conditions.

Model: DEAE Large Scale HP
Appearance: White spherical gel, odorless and tasteless
Type: Weak anion-exchange medium
Matrix: Large Scale HP microspheres
Ligand: Diethylaminoethyl
Particle size: Approximately 36-44 µm
Ovalbumin adsorption capacity: 90 mg/mL
Ion-exchange capacity: 0.26-0.32 mmol Cl-/mL
Flow performance: 220 cm/h at 0.3 MPa and 20 cm bed height
Operating temperature: 4-40°C
Long-term pH stability: 2-12
Short-term CIP pH stability: 1-14
Chemical compatibility: Common aqueous buffers, 1 mol/L sodium hydroxide, 8 mol/L urea, 8 mol/L guanidine hydrochloride, and 70% ethanol
Heat resistance: 121°C in water for 30 min

High-resolution 36-44 µm particles
Weak DEAE selectivity
90 mg/mL ovalbumin capacity
Low operating backpressure
Broad CIP pH range
Strong chemical stability
Steam-resistant agarose matrix
Suitable for process-scale purification

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