Cross-Link Immunopreciptiation Kit, 50 Reactions, 786-639, G-BIOSCIENCES (CAT#: STEM-GT-3023-LGZ)

Immunoprecipitation (IP) is one of the most useful immunochemical techniques available today. IPs are commonly used to determine the presence and amount of antigens, molecular weight of polypeptides, rates of synthesis or degradation, recognition of certain post-translational modifications, and interactions with other proteins, nucleic acids, and ligands. IPs consist of four main steps:

1. Antigen labeling (optional step).
2. Release of antigen by cell lysis.
3. Formation of antibody-antigen complexes.
4. Purify immune complexes.

G-Biosciences Crosslink Immunoprecipitation Kit contains all reagents needed to accomplish all aspects of immunoprecipitation, except labeling. Based on our classic immunoprecipitation kit, the cross-linked immunoprecipitation kit contains the homobifunctional cross-linker DSS to covalently couple the antibody to the immobilized protein A/G resin. This additional cross-linking step prevents contamination of the purified antigen with antibodies. The kit is designed for efficient immunoprecipitation using <10 µg of antibody. The kit is suitable for 50 reactions using 10 μl of immobilized protein A/G agarose.

Size: 50 Reactions

Eliminate Antibody Co-elution. Antibody is covalently coupled to the immobilized Protein A/G.

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