CM Seplife XL Weak Cation-Exchange Medium, 85 mg Lysozyme/mL (CAT#: STEM-BC-3157-ZJF)

This weak cation-exchange medium is designed for purification of proteins, nucleic acids, and peptides. It uses highly crosslinked agarose microspheres functionalized with carboxymethyl groups. The weak-acid ligand provides pH-dependent cation-exchange selectivity. A lysozyme adsorption capacity of 85 mg/mL supports preparative downstream purification. The medium combines high linear flow with relatively low backpressure. Low nonspecific adsorption contributes to product recovery and consistent performance. Broad chemical compatibility supports common buffers, alkaline cleaning, denaturants, and ethanol. The matrix tolerates the specified steam treatment in water. The resin is suitable for scalable capture, intermediate purification, and polishing operations.

Model: CM Seplife XL
Appearance: White spherical gel, odorless and tasteless
Type: Weak cation-exchange medium
Matrix: Seplife XL microspheres
Ligand: Carboxymethyl
Particle size: 45-165 µm
Lysozyme adsorption capacity: 85 mg/mL
Ion-exchange capacity: 0.10-0.14 mmol/mL
Maximum flow rate: 750 cm/h
Pressure resistance: 0.3 MPa
Operating temperature: 4-30°C
Long-term pH stability: 4-13
Short-term CIP pH stability: 2-14
Chemical compatibility: Common aqueous buffers, 1 mol/L sodium hydroxide, 8 mol/L urea, 8 mol/L guanidine hydrochloride, and 70% ethanol
Heat resistance: 121°C in water for 30 min

Weak carboxymethyl exchange
85 mg/mL lysozyme capacity
Up to 750 cm/h flow
Low operating backpressure
Broad chemical resistance
Steam-tolerant matrix
Low nonspecific adsorption
Suitable for process-scale purification

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