Butyl-S Seplife FF Hydrophobic Interaction Medium, 250-400 cm/h (CAT#: STEM-BC-3138-ZJF)

This hydrophobic interaction medium is designed for purification of recombinant hepatitis B vaccine products. It uses a six-percent agarose matrix functionalized with thiobutyl groups. The ligand provides hydrophobic binding with lower nonspecific adsorption than stronger hydrophobic media. Compatible target molecules can bind and elute at relatively low salt concentrations. This behavior supports processing of molecules with comparatively strong hydrophobic character. Spherical particles provide uniform packing and reproducible chromatography. Protein adsorption above 26 mg HSA/mL supports preparative purification. The medium operates at 250-400 cm/h under the stated pressure condition. Broad pH and chemical stability permit repeated cleaning, regeneration, and steam treatment.

Model: Butyl-S Seplife FF
Appearance: White spherical gel
Matrix: Seplife 6FF
Ligand: Thiobutyl
Shape: Spherical
Particle size: 45-165 µm
Pressure-flow range: 250-400 cm/h at 0.1 MPa
Protein adsorption capacity:>26 mg HSA/mL
Operating temperature: 4-40°C
Long-term pH stability: 3-13
Short-term CIP pH stability: 2-14
Chemical compatibility: Common aqueous buffers, 1 mol/L sodium hydroxide, 70% ethanol, 50% ethylene glycol, 8 M urea, 6 mol/L guanidine hydrochloride, and 1 mM hydrochloric acid
Heat resistance: 121°C in 0.1 M sodium chloride for 30 min

Thiobutyl hydrophobic ligand
Low nonspecific adsorption
Lower-salt binding and elution
250-400 cm/h flow
>26 mg/mL HSA capacity
Steam-resistant matrix
Wide CIP pH tolerance
Designed for recombinant vaccine purification

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