Butyl Large Scale HP Hydrophobic Interaction Medium, ~37 mg HSA/mL (CAT#: STEM-BC-3139-ZJF)

This high-performance hydrophobic interaction medium is designed for downstream purification of biological macromolecules. It uses small high-flow agarose microspheres functionalized with butyl ligands. The butyl groups provide hydrophobic selectivity under controlled salt conditions. Biomolecules bind according to exposed hydrophobic regions and elute as ionic strength decreases. The approximately 40 µm particles improve mass transfer and separation resolution. A dynamic HSA capacity of approximately 37 mg/mL supports productive preparative operation. The medium operates above 220 cm/h at the stated pressure and bed height. Broad pH stability supports repeated cleaning-in-place. It tolerates the listed alkaline solutions, alcohols, detergents, denaturants, and ammonium sulfate.

Model: Butyl Large Scale HP
Appearance: White spherical gel, odorless and tasteless
Type: Strong hydrophobic interaction medium
Matrix: Large Scale HP microspheres
Ligand: Butyl
Median particle size d50v: Approximately 40 µm
Dynamic binding capacity QB10: Approximately 37 mg HSA/mL
Operating temperature: 4-30°C
Flow performance:>220 cm/h at 0.3 MPa differential pressure and 20 cm bed height
Long-term pH stability: 3-13
Short-term CIP pH stability: 2-14
Chemical compatibility: 1 mol/L sodium hydroxide, 70% ethanol, 30% isopropanol, 0.5% SDS, 10% ethylene glycol, 6 mol/L guanidine hydrochloride, 8 mol/L urea, and 3 mol/L ammonium sulfate

High ~37 mg/mL HSA capacity
Fine 40 µm particles
Butyl hydrophobic selectivity
High-resolution separation
Broad chemical compatibility
Wide CIP pH range
Rigid high-flow agarose
Suitable for polishing workflows

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