BD FACSCelesta Cell Analyzer, BD Biosciences (CAT#: STEM-CAPO-0050-ZJF)

The BD FACSCelesta Cell Analyzer is manufactured by BD Biosciences.
The BD FACSCelesta are configured to have blue (488-nm) and violet (405-nm) lasers, which can be paired with a red (640-nm), ultraviolet (355-nm), or yellow-green (561-nm) laser, depending on your application needs. This unit has the ability to detect and analyze up to 14 parameters.
The BD FACSCelesta was manufactured in 2018 and is equipped with three lasers (red/blue/violet) with a NEW HTS. The unit recently had a PM by BD which passed all OEM specifications. The instrument comes with a computer loaded with DIVA software and a fluidics cart.

Year: 2018
Condition: Certified pre-owned
Excitation Optical Platform:
The BD FACSCelesta optics layout accommodates two or three lasers in one of four configurations.
BV10: 4 Blue (488 nm)/ 6 Violet (405 nm)
BVR12: 4 Blue (488 nm)/ 3 Red (640 nm)/ 5 Violet (405 nm)
BYV12: 2 Blue (488 nm)/ 4 Yellow-Green (561 nm)/ 6 Violet (405 nm)
BUV12: 4 Blue (488 nm)/ 2 UV (355 nm)/ 6 Violet (405 nm)
Laser Power:
355 nm: 15 mW
405 nm: 50 mW
488 nm: 20 mW
561 nm: 50 mW
640 nm: 40 mW
Operating Modes:
Front button panel provides three modes: RUN, STANDBY, and PRIME.
Sample Flow Rates:
Continuously adjustable flow rate, plus three preset flow rates:
LO: 12 μL/min
MED: 35 μL/min
HI: 60 μL/min
Fluorescence Sensitivity:
FITC: 25 molecules of equivalent soluble fluorochrome (MESF)
PE: 15 MESF
FITC and PE measurements were performed using SPHERO™ Rainbow Calibration Particles (RCP-30-5A).
Fluorescence Resolution:
Coefficient of variation: Area of <3%, full G0 /G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
Fluorescence Linearity:
Doublet/singlet ratio of 1.95–2.05 for CEN stained with PI and excited with the 488-nm blue laser
Forward and Side Scatter Sensitivity:
Enables separation of fixed platelets from noise
Forward and Side Scatter Resolution:
Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.
Side Scatter Resolution:
Enables separation of 0.3-μm beads from noise
Data Acquisition Rate:
25,000 events/s with beads

The BD FACSCelesta™ Cell Analyzer enables deep and powerful insights in cell analysis at your benchtop.
The BD FACSCelesta™ Cell Analyzer is optimized for use with BD Horizon Brilliant™ Dyes.
Options to enhance and tailor your BD FACSCelesta™ Cell Analyzer toward your specific research needs.

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