Bacillus Subtilis Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-1120-ZJF)

This kit utilizes SYBR Green dye-based real-time fluorescent PCR technology. Specific primers are designed to target highly conserved regions of the Bacillus Subtilis gene. In the presence of the target nucleic acid template, PCR amplification occurs, generating fluorescence signals. The fluorescence intensity is monitored in real time, enabling qualitative and quantitative analysis of Bacillus Subtilis with high sensitivity and specificity.

Kit Components:
Bacillus Subtilis Amplification Mix: 50 µL × 1 tube
Bacillus Subtilis Reaction Mix: 950 µL × 1 tube
Bacillus Subtilis Positive Control: 50 µL × 1 tube
Bacillus Subtilis Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Easy-start kit: DNA template is the only requirement.
Conserved-region targeting ensures reliable specificity.
Optimized formulation supports high-sensitivity detection.
Positive sample control included to validate results.
Self-contained PCR setup prevents external contamination.
Restricted to experimental research usage.

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