Avian Pathogenic Escherichia Coli O1 Serotype Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-1144-ZJF)

This real-time PCR kit incorporates SYBR Green dye chemistry for the detection ofAvian Pathogenic Escherichia Coli O1 Serotype. With primers tailored to conserved gene regions, the reaction generates fluorescence during amplification. The system monitors this signal continuously, allowing users to obtain precise qualitative and quantitative data from various sample types.

Kit Components:
Avian Pathogenic Escherichia Coli O1 Serotype Amplification Mix: 50 µL × 1 tube
Avian Pathogenic Escherichia Coli O1 Serotype Reaction Mix: 950 µL × 1 tube
Avian Pathogenic Escherichia Coli O1 Serotype Positive Control: 50 µL × 1 tube
Avian Pathogenic Escherichia Coli O1 Serotype Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Supplied in a ready-to-use format—just add DNA template.
Primers designed from conserved regions for maximum target specificity.
High sensitivity ensured through optimized primer/probe design.
Includes positive control to distinguish false negatives.
Single-tube fluorescence PCR minimizes contamination risk.
For research use only.

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