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Applied Biosystems 7500 Real Time PCR System, 100-240VAC, 12.5 Amps (CAT#: STEM-PCREPO-0033-YJL)

Highlights

It is validated for use with the Quantifiler™ Human Quantification and the Quantifiler™ Y Human Male DNA Quantification Systems.
It utilizes the Quantifiler™ kits to determine the quantity of amplifiable DNA and the potential presence of PCR inhibitors to obtain an accurate prediction of the amount of DNA input required for optimal STR amplification.
It utilizes the Quantifiler™ kits to quantify trace samples; real-time PCR has the widest dynamic range of any DNA detection technology.

Cat Number: STEM-PCREPO-0033-YJL

Application: Used to amplify DNA and RNA samples by the polymerase chain reaction

Model: 4345241R

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Description

Applied Biosystems 7500 Real Time PCR System, 100-240VAC, 12.5 Amps, model 4345241R, is manufactured by Applied Biosystems.
The Applied Biosystems 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes. The specially designed Peltier-based 7500 Fast block ensures thermal uniformity at top speeds. Faster ramp rates and novel well design enable rapid results without compromising extension times or assay quality.

Specification

Condition: Used
Electrical: 100-240VAC, 50/60hz, 1080W, 12.5 Amps
Format: 96-well Plate
Thermal Accuracy: 0.25°C (35°C to 95°C after 3 min.)
Sample Ramp Rate: ± 1.1°C⁄s
Sensitivity: 1 Copy of RNase P gene (Human Genomic DNA)
Temperature Range (Metric): 4 to 100°C
Reaction Volume Range: 20-100 µl
Passive Reference Dye: ROX (Separate Tube), No ROX, ROX (Pre-mixed)
Dynamic Range: 9 logs
Calibrated Dye: SYBR™ Green I, ROX™, NED™, TAMRA™, JOE™, Texas Red™, Cy5™, VIC™, FAM™, Cy3™
High-throughput Compatibility: Multiplexing
For Use With (Equipment): 7500 System
Thermal Uniformity: 0.50°C (after 30 s)
Reaction Speed: Standard
Throughput: 4 to 5-96-well Plates per 8-hr day
Optics: CCD Camera, 5 Excitation Filters, Tungsten-halogen Lamp, 5 Emission Filters
Thermal Cycling System: Peltier-based System
Dimensions: Width 34 cm (13.39 in.), Depth 45 cm (17.72 in.), Height 49 cm (19.29 in.)
Weight (English): 75 lb.
External Computer: 16XDVD+/-RW, 80 GB HD, 1 GB RAM, Pentium IV 630/3 GHz, Dell OptiPlex GX620 Minitower
Software Compatibility: GeneMapper™ ID v3.2, 7500 System Software, HID Real Time PCR Analysis Software v1.1
Precision: 99.7% Confidence (5000 & 10000 Copies)
Voltage: 120 V
Electrical Requirements: 950 W
Product Line: Primer Express™
Peak Block Ramp Rate: 2.5°C⁄s

Features

It is validated for use with the Quantifiler™ Human Quantification and the Quantifiler™ Y Human Male DNA Quantification Systems.
It utilizes the Quantifiler™ kits to determine the quantity of amplifiable DNA and the potential presence of PCR inhibitors to obtain an accurate prediction of the amount of DNA input required for optimal STR amplification.
It utilizes the Quantifiler™ kits to quantify trace samples; real-time PCR has the widest dynamic range of any DNA detection technology.
It provides Quantfiler™ kit results in less than 2 hours.

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