APC/C Signaling Pathway Assay (CAT#: STEM-MB-0314-WXH)

APC/C regulates the cell cycle primarily through collaboration with CDC20 and CDH1. The APC/C-CDC20 complex primarily regulates the degradation of related substrates in the mid-mitotic stages. The APC/C-CDH1 complex primarily regulates the progression of late mitosis and G1 phase. CDC20 plays an important role in the G2 phase of progress. In the G2 phase, CDC20 is phosphorylated by a kinase such as CDK1 (cyclin-dependent kinase 1), partially activating its interaction with APC/C. At the G2/M phase transition, the activity of the APC/C-CDC20 complex is inhibited. In the middle stage of mitosis, the APC/C-CDC20 complex can ubiquitinate and degrade Cyclin A, and NEK2A (NIMA-related expressed kinase 2A).

To study the regulation mechanism of APC/C signal pathway in disease.
To study the effect of each virus on APC/C signaling pathway.
To study the effects of drugs or therapies on APC/C signaling pathways.

• Luminex Multiplex Assay
• Enzyme-linked immunosorbent assay (ELISA)
• Flow cytometry (FACS analysis) technology

Detectable targets: p53, BID, APs, tBID, SODD, Bad, IPS-1, ISGF3, MSK2, PI3K, TBK1, Vav, BAK, Bax, PIDD, MYD88, Rac1, TLR4, Histone-H3, IRF5, MEK3, p38MAPK, SH2, TRAF6, Bcl-xl, RIP1, TRAF-2, FADD, APAF-1, BID, IKK-α, IRS1, MEK6, p50, SLP76, TRAM, FADD, IRF7, Mda-5, NFκB, RIG-1, TRAF3, IKK-β, IRS2, MSK1, p65, Tak1, TRIF, GAS, IRF9, MEKK1, p38, RIP1, TRAF5, IRAK1, ISRE, mTOR, PKR, TLR3