Analysis of ZFP36L2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1740-LGZ)

Name: Analysis of ZFP36L2 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1740-LGZ
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Introduction

Official Full Name: ZFP36 ring finger protein like 2
Also known as: BRF2; ERF2; ERF-2; OOMD13; TIS11D; OZEMA13; RNF162C
This gene is a member of the TIS11 family of early response genes. Family members are induced by various agonists such as the phorbol ester TPA and the polypeptide mitogen EGF. The encoded protein contains a distinguishing putative zinc finger domain with a repeating cys-his motif. This putative nuclear transcription factor most likely functions in regulating the response to growth factors.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.